STEM-19. LIVE DETECTION OF NEURAL STEM AND GLIOBLASTOMA CELLS BY A LUMINESCENT CONJUGATED OLIGOTHIOPHENE DERIVATIVE

Abstract Glioblastoma (GBM) is an aggressive nervous system tumor with a mean survival time of 12-14 months. Cells neural stem cell-like properties can be derived from GBM tumors. These cells seem to escape conventional irradiation treatment, chemotherapy, and surgery, may play crucial role for relapse. It therefore urgent develop novel approaches reliable detection in GBM. Here we report luminescent conjugated oligothiophene (LCO), named GlioStem (p-HTMI), non-invasive non-amplified real-time live human patient-derived embryonic stem/progenitor (NSPCs). Within maximum 10 minutes after administration the molecule vitro, existing media, fluorescence emission was observed without any modulation or additional vehicle, resulting efficient cytoplasmic signal NSPCs rodents humans, detectable at Alexa488/GFP wavelength. functionalized methylated imidazole moiety resembling side chain histidine/histamine, non-methylated analogues were not functional. In shown identify fetal cortical rat (FGF2-expanded), cell-derived mouse (FGF2/EGF-expanded), FGF2-exposed C6 glioma cell cultures rat, but other types investigated. Cell sorting experiments patient-derived, FGF2/EGF-expanded demonstrated that addition NSPC-markers like Nestin Sox2 labeled same population (overlap > 90%) as CD271, proposed marker rapidly migrating glioblastoma. Our results suggest LCO versatile tool immediate selective subpopulations cells.